NRF2 Is a Potential Modulator of Hyperresistance to Arsenic Toxicity in Stem-Like Keratinocytes
High NRF2 activity and ROS levels in CD34high-enriched cells. (a) Representative image of Western blot for NRF2 in CD34low-expressing, CD34high-enriched, and parent cells. Cells were exposed to Veh. or 20 μM sodium arsenite for 6 h. (b) Innate mRNA levels of NRF2 downstream genes, including those encoding MRPs and antioxidant-related proteins in CD34low-expressing, CD34high-enriched, and parent cells. (c) Quantification and (d) representative histogram for intracellular ROS determined with flow cytometry. Cells were treated with Veh., 20 μM, or 40 μM sodium arsenite for 2 h. . compared with CD34low-expressing compartment. # compared with parent compartment.