Oxygen-Sensing Nox4 Generates Genotoxic ROS to Induce Premature Senescence of Nucleus Pulposus Cells through MAPK and NF-κB Pathways

Figure 2

High oxygen tension induced premature senescence of NP cells through ROS/oxidative stress. (a, b) Quantitative PCR analysis () and representative immunoblot analysis of p53, p16, p21, Rb, and p-Rb in high oxygen tension-treated NP cells. (c) The percentage of SA-β-gal-positive NP cells (). (d, e) Immunofluorescence staining of BrdU and percentage of BrdU-positive cells in high oxygen tension-treated NP cells (). (f, g) RT-qPCR analysis of matrix proteases and proinflammatory cytokines in high oxygen tension-treated NP cells (). NP cells were pretreated with GSH and NAC for 30 min followed by high oxygen tension treatment for ROS scavenging. , value < 0.05, error bars represent standard error.

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