Oxygen-Sensing Nox4 Generates Genotoxic ROS to Induce Premature Senescence of Nucleus Pulposus Cells through MAPK and NF-κB Pathways

Figure 6

Nox4 overexpression boosted ROS production and induced DNA damage in NP cells. (a) RT-qPCR analysis () of Nox4, MsrB1, and MsrB2 in NP cells overexpressing Nox4. (b) Representative immunoblot analysis of Nox4 in NP cells overexpressing Nox4. (c) ROS production in NP cells overexpressing Nox4 (). (d, e) Immunofluorescence staining of γ-H2A.X and percentage of γ-H2A.X-positive cells in NP cells overexpressing Nox4 (). NP cells were transfected with Nox4 vectors for Nox4 overexpression. , value < 0.05, error bars represent standard error.

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