Oxidative Medicine and Cellular Longevity / 2017 / Article / Fig 6

Research Article

A Carotenoid Extract from a Southern Italian Cultivar of Pumpkin Triggers Nonprotective Autophagy in Malignant Cells

Figure 6

Chloroquine and bafilomycin A1 inhibit autophagy. Autophagosome quantification expressed as FITC/DAPI fluorescence ratio with Cyto-ID kit in SAOs treated with 20 μM chloroquine (a) or 50 nM bafilomycin A1 (b) for 48 h. Bar graphs represent the mean ± SD with symbols indicating significance: and with respect to untreated cells. (c) Immunoblotting showing LC3-I/LC3-II expression in SAOs cells treated with bafilomycin A1 as indicated above. Densitometric analysis values (numbers between panels) were expressed by the ratio LC3-II/α-tubulin.

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