Oxidative Medicine and Cellular Longevity / 2017 / Article / Fig 5

Research Article

Resveratrol Prevents ROS-Induced Apoptosis in High Glucose-Treated Retinal Capillary Endothelial Cells via the Activation of AMPK/Sirt1/PGC-1α Pathway

Figure 5

Effects of RSV on the HG-stimulated AMPK-SIRT1-PGC-1α axis in BRECs. (a, b) Sirt1 and PGC-1α mRNA expression were quantified by real-time RT-PCR in BRECs. (c) Representative Western blot analysis of AMPK, phospho-AMPK Thr172, Sirt1, PGC-1α, and GADPH levels. (d–g) Bars indicate the mean ± SEM from three independent experiments. GADPH was set as a control for normalization (). versus NG, # versus HG-treated group, versus HG plus RSV-treated group.
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