Resveratrol Prevents ROS-Induced Apoptosis in High Glucose-Treated Retinal Capillary Endothelial Cells via the Activation of AMPK/Sirt1/PGC-1α Pathway

The role of AMPK-SIRT1-PGC-1α in RSV treatment of HG-induced BREC apoptosis. (a) Annexin V-FITC/PI staining of BRECs in each experimental group. (b) Percent of cells that were PI positive. (c) Western blot analysis for cleaved caspase-3. (d) Western blot quantification. Caspase-3 levels were normalized by GADPH, and data are expressed relative to the NG mean. Bars indicate the mean ± SEM of three independent experiments. versus NG, ^# versus HG-treated group, ^† versus HG plus RSV-treated group.