RTA-408 Protects Kidney from Ischemia-Reperfusion Injury in Mice via Activating Nrf2 and Downstream GSH Biosynthesis Gene

Figure 5

Effect of RTA-408 treatment on Nrf2 and Nrf2 target gene activation. (a, b) Western blot analysis of cytoplasm lysate and nucleus lysate from injured kidney at 24 h reperfusion (; ^#). Nuclear translocation of Nrf2 by RTA-408 was measured by immunohistochemistry staining (). (c, d) Black arrows indicate Nrf2-translocated nuclei (magnification 400x; scale bar 50 μm). The expression intensity quantification was measured by software and shown as a bar graph. (e, f) Western blot analysis showed that Nrf2 downstream genes HO-1 (), NQO-1 (), GSR (), and GCLc () were notably induced by RTA-408. All data were expressed as mean ± SD. ^/#Significant difference versus the vehicle + I/R group.

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