Acarbose Accelerates Wound Healing via Akt/eNOS Signaling in db/db Mice
Acarbose alleviated EPC dysfunction, decreased ROS expression, and increased Akt/eNOS phosphorylated-to-total ratio in BM-EPCs induced by high glucose. BM-EPCs were isolated from normal mice and cultured under high glucose (33 mM) together with acarbose (1 μM) for 24 h. (a) Assessment of tube formation ability. (b) Intracellular NO level was determined by flow cytometry and the percentage of DAF fluorescence intensity was calculated. (c) Intracellular DHE fluorescence intensity of EPCs was measured by flow cytometry. Western blot analysis was subjected to detected expression levels of activation Akt (d) and eNOS (e) in EPCs induced by high glucose. , , and versus Con; , versus HG. Values are expressed as the mean ± standard deviation ((a), (b), (c): per group; (d), (e): -4 per group). Scale bar = 100 μm. Con, control; HG, high glucose; Aca, acarbose.
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