Mulberry Anthocyanin Extract Ameliorates Oxidative Damage in HepG2 Cells and Prolongs the Lifespan of Caenorhabditis elegans through MAPK and Nrf2 Pathways

Figure 2

Effect of MAE on cytoprotection and glucose metabolism in H₂O₂-induced HepG2 cells. (a) Cell viability analyzed by MTT assays; (b) HepG2 cells were incubated with 5, 25, 50, 100, and 250 μg/mL MAE or 1 mM GSH for 24 h and were exposed to H₂O₂ (300 μM, 4 h). After that, all media were removed and changed to the same for glucose consumption measurement. Vertical lines represent standard deviations of five replicates. (c) Uptake of 2-NBDG into HepG2 cells. (A) control, (B) H₂O₂ (300 μM, 4 h), (C) GSH (1 mM), and (D) MAE (100 μg/mL). Densitometry analysis was performed using the Image-Pro Plus 6.0 software. Values with different letters above are significantly different, , one-way ANOVA test.

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