Mulberry Anthocyanin Extract Ameliorates Oxidative Damage in HepG2 Cells and Prolongs the Lifespan of Caenorhabditis elegans through MAPK and Nrf2 Pathways

Table 1

Quantification of anthocyanins, protein, and total soluble carbohydrate contents in MAE.

Fraction	Retention time (min)	Content (mg/g)
Cyanidin-3-glucoside	31.2	472 ± 9.23
Cyanidin-3-rutinoside	35.7	273 ± 5.12
Pelargonidin-3-glucoside	38.0	14 ± 0.29
Protein	—	72 ± 2.8
Total soluble carbohydrate	—	86 ± 3.6

Anthocyanin concentrations were determined by HPLC analysis. Protein content was determined by the Bradford method and measured at 595 nm. Total soluble carbohydrate was determined by the phenol-sulfuric acid method and measured at 490 nm. All values are mean ± SD. Three replicates were performed in the analysis.