The Neuroprotective Effects of Brazilian Green Propolis on Neurodegenerative Damage in Human Neuronal SH-SY5Y Cells

Figure 5

The effect of methanol extracts of propolis on BDNF expression in SH-SY5Y cells. (a) Time-dependent effects of 10 μg/mL propolis on BDNF mRNA expression in SH-SY5Y cells. (b) Dose-dependent effects of propolis on Arc mRNA expression in SH-SY5Y cells at 60 min after incubation. (c) Effects of specific inhibitors on BDNF mRNA expression. GF109203X hydrochloride (protein kinase C inhibitor, 200 μM), Y-27632 dihydrochloride (Rho-associated protein kinase inhibitor, 1 μM), and Wortmannin (phosphoinositide 3-kinase inhibitor, 200 nM) were pretreated for 1 h, followed by incubation with 10 μg/mL propolis for 30 min. (d) The expression of Arc after treatment with Carbachol (1 mM) and pretreatment with U0126 (ERK inhibitors, 10 μM) or ANA-12 (TrkB selective antagonist, 100 nM). (e) The quantitative analysis of Arc protein expression shown in (d). The results are expressed as the mean ± SEM ( each), and the asterisks indicate a statistically significant difference from the indicated value (, , and ).

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