Effect of Whole-Body Cryotherapy on Antioxidant Systems in Experimental Rat Model

Table 2

Nonenzymatic antioxidant system. The concentration of lipid peroxidation product: malondialdehyde (MDA) in plasma, haemolisates, and liver; total antioxidant capacity (TAC) in plasma and concentration of uric acid (UA) in serum of rats after WBC.

Temperature (°C):		−60		−90		ANOVA	Multiple comparison with control
Time:		5	10	5	10		A	B	C	D
	Control	Group A	Group B	Group C	Group D
MDA; [mmol/L plasma]	2.49; (2.35–2.66)	2.63; (2.62–2.65)	2.63; (2.61–2.65)	4.12; (4.01–4.14)	4.15; (4.13–4.65)	<0.001	0.246	0.257	<0.001	<0.001
MDA; [μmol/g Hgb]	355.8 ± 11.0	319.5 ± 13.4	315.0 ± 23.6	349.0 ± 6.9	402.3 ± 24.4	<0.001	<0.01	<0.01	0.898	<0.001
MDA [μmol/g protein]	330.8 ± 18.1	317.5 ± 12.5	339.2 ± 11.7	402.2 ± 6.7	315.3 ± 16.0	<0.001	0.285	0.672	<0.001	0.177
TAC [mmol/L]	2.03 ± 0.02	3.40 ± 0.21	2.90 ± 0.05	1.67 ± 0.09	1.76 ± 0.12	<0.001	<0.001	<0.001	<0.001	<0.01
UA [μmol/L]	76.48 ± 2.92	67.82 ± 9.81	90.60 ± 8.82	55.40 ± 2.63	63.65 ± 2.36	<0.001	0.077	<0.01	<0.001	<0.01

Descriptive statistics and results of one-way analysis of variance. Values were expressed as a mean value ± standard deviation or as median/interquartile range in the case of data with skewed or nonnormal distribution. Statistical significance was set at p < 0.05. All groups are compared to the control one. Control: animals that did not undergo WBC treatment; Group A −60/5: 1 min of WBC stimulation, 5 days, −60°C; Group B −60/10: 1 min of WBC stimulation, 10 days, −60°C; Group C −90/5: 1 min of WBC stimulation, 5 days, −90°C; Group D −90/10: 1 min of WBC stimulation, 10 days, −90°C.