Research Article
Enhancement of Mitochondrial Transfer by Antioxidants in Human Mesenchymal Stem Cells
Figure 3
MCA enhanced TNT formation after coculture. TNTs decreased when microtubule-disrupting agent nocodazole (50 μM) was included in the coculture system (a). The number of TNTs were enumerated and classified per 100 cells, and the proportion of TNTs that connected multiple cells increased from 34% to 44% after MCA treatment (b). Nocodazole almost disrupted multiple connected-TNTs in the coculture system. A representative image of long TNT after MCA treatment in the coculture system is shown in (c). The average length of TNTs formed in coculture containing H2O2-treated hMSCs and hMSCs with or without MCA were 53.5 μm and 28.5 μm, respectively (d). TNTs that were more than 50 μm in length was 35.7% in the coculture containing MCA-treated and H2O2-treated hMSCs as compared to only 8.6% in the non-MCA-treated hMSCs coculture (e). , as compared to the non-MCA-treated hMSCs coculture. Scale bar: 20 μm.
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