Enhancement of Mitochondrial Transfer by Antioxidants in Human Mesenchymal Stem Cells

Figure 6

Maintenance of mitochondrial membrane potential (ΔΨm) after coculture with MCA-hMSCs. (a) The ΔΨm was measured using JC-1 in hMSCs coculture system. The JC-1 was mostly red (indicating higher ΔΨm) in coculture containing MCA-treated hMSCs (right panel), and the mitochondria appeared to be longer as compared to the control hMSCs coculture (comparing the enlarged insets from the right and left panels). (b) The cocultured cells were analyzed with flow cytometry. The proportion of cells with lower ΔΨm (green fluorescence) decreased in coculture containing MCA-treated hMSCs. The rescue of ΔΨm in the coculture decreased significantly in coculture medium containing nocodazole (c). .

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