Enhancement of Mitochondrial Transfer by Antioxidants in Human Mesenchymal Stem Cells

Figure 8

Mitochondria transfer helped regulating mitochondria quality in the injured hMSCs. (a) The mitochondria from the H₂O₂-hMSCs were labeled with MitoTracker Green, while the mitochondria from the healthy hMSCs were labeled with MitoTracker Red, respectively, before 6 h coculture. The number of MitoTracker Red-labeled mitochondria in the recipients increased in coculture containing MCA-treated hMSCs, while the MitoTracker Green-labeled mitochondria were shorter and tended to form aggregates (arrowheads in the enlarge insets). (b) Both cocultured cells were stained with MitoTracker Green and LysoRed to identify the fate of the short mitochondria. Many mitochondrial aggregates in the coculture containing MCA-treated hMSCs colocalized with LysoRed-labeled lysosomes (arrowheads in the enlarged insets of the right panel). (c) Fluorescence intensity analysis of the respective insets in (b) confirmed that the green fluorescence was in the same plane of the red fluorescence. Scale bar: 20 μm.

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