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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 8530656, 10 pages
Research Article

Selaginella bryopteris Aqueous Extract Improves Stability and Function of Cryopreserved Human Mesenchymal Stem Cells

1Department of Biochemistry, University of Allahabad, Allahabad 211002, India
2Department of Biotechnology, National Institute of Technology, Raipur 492010, India
3Department of Biomedical Engineering, National Institute of Technology, Raipur 492010, India
4Kazan Federal University, Kazan, Russia
5Shona Lab, Centre for Brain Development and Repair, InStem National Centre for Biological Sciences, Bangalore, Karnataka 560065, India
6Department of Biochemistry, Pt. JNM Medical College, Raipur 492001, India

Correspondence should be addressed to Awanish Kumar; and Akalabya Bissoyi; moc.liamg@aybalaka.iyossib

Received 11 April 2017; Revised 2 June 2017; Accepted 15 June 2017; Published 24 July 2017

Academic Editor: Nageswara Madamanchi

Copyright © 2017 Abhishek Kumar Singh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The effective long-term cryopreservation of human mesenchymal stem cells (MSCs) is an essential prerequisite step and represents a critical approach for their sustained supply in basic research, regenerative medicine, and tissue engineering applications. Therefore, attempts have been made in the present investigation to formulate a freezing solution consisting of a combination of Selaginella bryopteris water-soluble extract with and without dimethyl sulfoxide (Me2SO) for the efficient long-term storage of human umbilical cord blood- (hUCB-) derived MSCs. The cryopreservation experiment using the formulated freezing solution was further performed with hUCB MSCs in a controlled rate freezer. A significant increase in postthaw cell viability and cell attachment of MSCs was achieved with freezing medium containing Selaginella bryopteris water extract along with 10% Me2SO as compared to the freezing medium containing Me2SO (10% v/v) alone. Furthermore, the decreasing apoptotic events and reactive oxygen species production along with increasing expression of heat shock proteins also confirmed the beneficial effect of Selaginella bryopteris water extract. The beneficial effect of Selaginella bryopteris water extract was validated by its ability to render postpreservation high cell viability. In conclusion, the formulated freezing solution has been demonstrated to be effective for the standardization of cryopreservation protocol for hMSCs.