Research Article
Activation of p47phox as a Mechanism of Bupivacaine-Induced Burst Production of Reactive Oxygen Species and Neural Toxicity
Figure 4
p47phox gene knockdown reduced bupivacaine-induced ROS overexpression and cell injury. (a, b) The level of cytoplasmic peroxide (DCFH-DA, green) and O2.− (DHE, red) was measured by microscopy. (c) The production of cytoplasmic peroxide (DCFH-DA) and O2.− (DHE) was measured by a microplate reader. (d, e) The ratio of apoptotic cells was detected by TUNEL staining. Cells tagged by white arrows were TUNEL positive. (f, g) The protein level of cleaved caspase-3 and phospho-γ-H2A.x was measured by Western blot. (f, h) The translocation of p47phox was measured by Western blot. Data represent mean ± SD of at least 3 independent experiments. Scale bar: 50 μm. versus control (NC) group. # versus Bup group. & versus p47si group.
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