Overexpression of Brg1 Alleviates Hepatic Ischemia/Reperfusion-Induced Acute Lung Injury through Antioxidative Stress Effects

Figure 4

Overexpression of Brg1 protects against oxidative stress induced by HIR. (a, b) Lung 8-isoprostane and ROS levels. (c, d) Immunohistochemical staining (×200) of lung 4-hydroxynonenal (4-HNE) (brown) and densitometric analysis showed that the 4-HNE-positive cells were significantly reduced in the CMV-Brg1 mice than in the WT mice at 6 h after HIR. (e, f) Immunofluorescence staining (×200) of lung 8-hydroxy-2-deoxyguanosine (8-OHdG) and densitometric analysis showed that the relative fluorescence intensity was significantly decreased in the CMV-Brg1 mice compared to the WT mice at 6 h after HIR. The 8-OHdG-positive cells were stained red, and the nuclei of tissue sections were counterstained blue by 4,6-diamidino-2-phenylindole. ; .

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