Oxidative Medicine and Cellular Longevity / 2017 / Article / Fig 3

Research Article

Molecular Hydrogen Effectively Heals Alkali-Injured Cornea via Suppression of Oxidative Stress

Figure 3

The immunohistochemical detection of nitrotyrosine (NT) and malondialdehyde (MDA) in injured corneas treated with H2 dissolved in PBS (H2) or with PBS free of H2 (buffer) examined on day 20 after the injury. In buffer treated injured corneas the expressions of NT (a) and MDA (b) were high (arrows). This was in contrast to H2 solution treated injured corneas, where the expressions of NT (c) and MDA (d) were absent, similarly to in control corneas (e, f). Scale bars: 50 μm.

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