Molecular Hydrogen Effectively Heals Alkali-Injured Cornea via Suppression of Oxidative Stress
The immunohistochemical detection of vascular endothelial growth factor (VEGF) in injured corneas treated with H2 dissolved in PBS (H2) or with PBS free of H2 (buffer) examined on day 20 after the injury. In buffer treated corneas the expression of VEGF was high (a), (arrows) and corneas are vascularized, whereas the expression of VEGF in damaged corneas after H2 treatment was low (b) compared with control cornea (c). Scale bars: 50 μm. The expression of genes for VEGF determined by real-time PCR (d). In graphs the values with asterisks represent statistically significant () difference from injured buffer treated corneas.
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