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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 9076052, 10 pages
https://doi.org/10.1155/2017/9076052
Research Article

Oleic Acid and Hydroxytyrosol Inhibit Cholesterol and Fatty Acid Synthesis in C6 Glioma Cells

1Laboratory of Biochemistry and Molecular Biology, Department of Biological and Environmental Sciences and Technologies, University of Salento, Via Prov.le Lecce-Monteroni, 73100 Lecce, Italy
2CNR-NANOTEC, Institute of Nanotechnology c/o Campus Ecotekne, University of Salento, Via Monteroni, 73100 Lecce, Italy
3Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari “Aldo Moro”, Policlinico P.zza G. Cesare 11, 70100 Bari, Italy

Correspondence should be addressed to Luisa Siculella; ti.otnelasinu@allelucis.asiul

Received 26 May 2017; Revised 8 September 2017; Accepted 5 December 2017; Published 24 December 2017

Academic Editor: Tommaso Cassano

Copyright © 2017 Paola Priore et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. E. Toledo, J. Salas-Salvadó, C. Donat-Vargas et al., “Mediterranean diet and invasive breast cancer risk among women at high cardiovascular risk in the PREDIMED trial: a randomized clinical trial,” JAMA Internal Medicine, vol. 175, no. 11, pp. 1752–1760, 2015. View at Publisher · View at Google Scholar · View at Scopus
  2. M. Á. Martínez-González, M. Ruiz-Canela, A. Hruby, L. Liang, A. Trichopoulou, and F. B. Hu, “Intervention trials with the Mediterranean diet in cardiovascular prevention: understanding potential mechanisms through metabolomic profiling,” The Journal of Nutrition, vol. 146, no. 4, pp. 913S–919S, 2016. View at Publisher · View at Google Scholar · View at Scopus
  3. J. Rodríguez-Morató, L. Xicota, M. Fitó, M. Farré, M. Dierssen, and R. de la Torre, “Potential role of olive oil phenolic compounds in the prevention of neurodegenerative diseases,” Molecules, vol. 20, no. 3, pp. 4655–4680, 2015. View at Publisher · View at Google Scholar · View at Scopus
  4. L. Fernández del Río, E. Gutiérrez-Casado, A. Varela-López, and J. Villalba, “Olive oil and the hallmarks of aging,” Molecules, vol. 21, no. 2, p. 163, 2016. View at Publisher · View at Google Scholar · View at Scopus
  5. F. Natali, L. Siculella, S. Salvati, and G. V. Gnoni, “Oleic acid is a potent inhibitor of fatty acid and cholesterol synthesis in C6 glioma cells,” The Journal of Lipid Research, vol. 48, no. 9, pp. 1966–1975, 2007. View at Publisher · View at Google Scholar · View at Scopus
  6. P. Priore, A. Cavallo, A. Gnoni, F. Damiano, G. V. Gnoni, and L. Siculella, “Modulation of hepatic lipid metabolism by olive oil and its phenols in nonalcoholic fatty liver disease,” IUBMB Life, vol. 67, no. 1, pp. 9–17, 2015. View at Publisher · View at Google Scholar · View at Scopus
  7. E. Lei, K. Vacy, and W. C. Boon, “Fatty acids and their therapeutic potential in neurological disorders,” Neurochemistry International, vol. 95, pp. 75–84, 2016. View at Publisher · View at Google Scholar · View at Scopus
  8. M. Piroddi, A. Albini, R. Fabiani et al., “Nutrigenomics of extra-virgin olive oil: a review,” BioFactors, vol. 43, no. 1, pp. 17–41, 2017. View at Publisher · View at Google Scholar · View at Scopus
  9. R. Fabiani, “Anti-cancer properties of olive oil secoiridoid phenols: a systematic review of in vivo studies,” Food & Function, vol. 7, no. 10, pp. 4145–4159, 2016. View at Publisher · View at Google Scholar · View at Scopus
  10. C. C. Tangney and H. E. Rasmussen, “Polyphenols, inflammation, and cardiovascular disease,” Current Atherosclerosis Reports, vol. 15, no. 5, p. 324, 2013. View at Publisher · View at Google Scholar · View at Scopus
  11. F. Visioli, G. Bellomo, and C. Galli, “Free radical-scavenging properties of olive oil polyphenols,” Biochemical and Biophysical Research Communications, vol. 247, no. 1, pp. 60–64, 1998. View at Publisher · View at Google Scholar · View at Scopus
  12. P. Priore, L. Siculella, and G. V. Gnoni, “Extra virgin olive oil phenols down-regulate lipid synthesis in primary-cultured rat-hepatocytes,” The Journal of Nutritional Biochemistry, vol. 25, no. 7, pp. 683–691, 2014. View at Publisher · View at Google Scholar · View at Scopus
  13. P. Priore, D. Caruso, L. Siculella, and G. V. Gnoni, “Rapid down-regulation of hepatic lipid metabolism by phenolic fraction from extra virgin olive oil,” European Journal of Nutrition, vol. 54, no. 5, pp. 823–833, 2015. View at Publisher · View at Google Scholar · View at Scopus
  14. W. J. Lukiw, M. Pappolla, R. P. Pelaez, and N. G. Bazan, “Alzheimer’s disease—a dysfunction in cholesterol and lipid metabolism,” Cellular and Molecular Neurobiology, vol. 25, no. 3-4, pp. 475–483, 2005. View at Publisher · View at Google Scholar · View at Scopus
  15. A. Romano, J. B. Koczwara, C. A. Gallelli et al., “Fats for thoughts: an update on brain fatty acid metabolism,” The International Journal of Biochemistry & Cell Biology, vol. 84, pp. 40–45, 2017. View at Publisher · View at Google Scholar
  16. C. Féart, C. Samieri, V. Rondeau et al., “Adherence to a Mediterranean diet, cognitive decline, and risk of dementia,” JAMA, vol. 302, no. 6, pp. 638–648, 2009. View at Publisher · View at Google Scholar · View at Scopus
  17. V. Pitozzi, M. Jacomelli, M. Zaid et al., “Effects of dietary extra-virgin olive oil on behaviour and brain biochemical parameters in ageing rats,” British Journal of Nutrition, vol. 103, no. 11, pp. 1674–1683, 2010. View at Publisher · View at Google Scholar · View at Scopus
  18. D. Pantano, I. Luccarini, P. Nardiello, M. Servili, M. Stefani, and F. Casamenti, “Oleuropein aglycone and polyphenols from olive mill waste water ameliorate cognitive deficits and neuropathology,” British Journal of Clinical Pharmacology, vol. 83, no. 1, pp. 54–62, 2017. View at Publisher · View at Google Scholar
  19. S. Torres, M. Lorente, F. Rodríguez-Fornés et al., “A combined preclinical therapy of cannabinoids and temozolomide against glioma,” Molecular Cancer Therapeutics, vol. 10, no. 1, pp. 90–103, 2011. View at Publisher · View at Google Scholar · View at Scopus
  20. I. Matias, A. S. Buosi, and F. C. A. Gomes, “Functions of flavonoids in the central nervous system: astrocytes as targets for natural compounds,” Neurochemistry International, vol. 95, pp. 85–91, 2016. View at Publisher · View at Google Scholar · View at Scopus
  21. J. J. Volpe, K. Fujimoto, J. C. Marasa, and H. C. Agrawal, “Relation of C-6 glial cells in culture to myelin,” The Biochemical Journal, vol. 152, no. 3, pp. 701–703, 1975. View at Publisher · View at Google Scholar
  22. F. A. McMorris, “Norepinephrine induces glial-specific enzyme activity in cultured plasma glioma cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 74, no. 10, pp. 4501–4504, 1977. View at Publisher · View at Google Scholar
  23. K. A. Nave and G. Lemke, “Induction of the myelin proteolipid protein (PLP) gene in C6 glioblastoma cells: functional analysis of the PLP promotor,” The Journal of Neuroscience, vol. 11, no. 10, pp. 3060–3069, 1991. View at Google Scholar
  24. A. Quincozes-Santos, L. D. Bobermin, A. Latini et al., “Resveratrol protects C6 astrocyte cell line against hydrogen peroxide-induced oxidative stress through heme oxygenase 1,” PLoS One, vol. 8, no. 5, article e64372, 2013. View at Publisher · View at Google Scholar · View at Scopus
  25. G. V. Gnoni, M. J. H. Geelen, C. Bijleveld, E. Quagliariello, and S. G. van den Bergh, “Short-term stimulation of lipogenesis by triiodothyronine in maintenance cultures of rat hepatocytes,” Biochemical and Biophysical Research Communications, vol. 128, no. 2, pp. 525–530, 1985. View at Publisher · View at Google Scholar · View at Scopus
  26. E. G. Bligh and W. J. Dyer, “A rapid method of total lipid extraction and purification,” Canadian Journal of Biochemistry and Physiology, vol. 37, no. 1, pp. 911–917, 1959. View at Publisher · View at Google Scholar
  27. M. J. H. Geelen, “The use of digitonin-permeabilized mammalian cells for measuring enzyme activities in the course of studies on lipid metabolism,” Analytical Biochemistry, vol. 347, no. 1, pp. 1–9, 2005. View at Publisher · View at Google Scholar · View at Scopus
  28. P. Priore, A. M. Giudetti, F. Natali, G. V. Gnoni, and M. J. H. Geelen, “Metabolism and short-term metabolic effects of conjugated linoleic acids in rat hepatocytes,” Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, vol. 1771, no. 10, pp. 1299–1307, 2007. View at Publisher · View at Google Scholar · View at Scopus
  29. F. Damiano, R. Tocci, G. V. Gnoni, and L. Siculella, “Expression of citrate carrier gene is activated by ER stress effectors XBP1 and ATF6α, binding to an UPRE in its promoter,” Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, vol. 1849, no. 1, pp. 23–31, 2015. View at Publisher · View at Google Scholar · View at Scopus
  30. L. Siculella, R. Tocci, A. Rochira, M. Testini, A. Gnoni, and F. Damiano, “Lipid accumulation stimulates the cap-independent translation of SREBP-1a mRNA by promoting hnRNP A1 binding to its 5-UTR in a cellular model of hepatic steatosis,” Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, vol. 1861, no. 5, pp. 471–481, 2016. View at Publisher · View at Google Scholar · View at Scopus
  31. E. Scoditti, N. Calabriso, M. Massaro et al., “Mediterranean diet polyphenols reduce inflammatory angiogenesis through MMP-9 and COX-2 inhibition in human vascular endothelial cells: a potentially protective mechanism in atherosclerotic vascular disease and cancer,” Archives of Biochemistry and Biophysics, vol. 527, no. 2, pp. 81–89, 2012. View at Publisher · View at Google Scholar · View at Scopus
  32. E. Scoditti, A. Nestola, M. Massaro et al., “Hydroxytyrosol suppresses MMP-9 and COX-2 activity and expression in activated human monocytes via PKCα and PKCβ1 inhibition,” Atherosclerosis, vol. 232, no. 1, pp. 17–24, 2014. View at Publisher · View at Google Scholar · View at Scopus
  33. N. Scarmeas, Y. Stern, M. X. Tang, R. Mayeux, and J. A. Luchsinger, “Mediterranean diet and risk for Alzheimer’s disease,” Annals of Neurology, vol. 59, no. 6, pp. 912–921, 2006. View at Publisher · View at Google Scholar · View at Scopus
  34. R. W. Mitchell, N. H. On, M. R. Del Bigio, D. W. Miller, and G. M. Hatch, “Fatty acid transport protein expression in human brain and potential role in fatty acid transport across human brain microvessel endothelial cells,” Journal of Neurochemistry, vol. 117, no. 4, pp. 735–746, 2011. View at Publisher · View at Google Scholar · View at Scopus
  35. J. Zhang and Q. Liu, “Cholesterol metabolism and homeostasis in the brain,” Protein & Cell, vol. 6, no. 4, pp. 254–264, 2015. View at Publisher · View at Google Scholar · View at Scopus
  36. M. Madra and S. L. Sturley, “Niemann-Pick type C pathogenesis and treatment: from statins to sugars,” Clinical Lipidology, vol. 5, no. 3, pp. 387–395, 2010. View at Publisher · View at Google Scholar · View at Scopus
  37. N. Sato and R. Morishita, “The roles of lipid and glucose metabolism in modulation of β-amyloid, tau, and neurodegeneration in the pathogenesis of Alzheimer disease,” Frontiers in Aging Neuroscience, vol. 7, p. 199, 2015. View at Publisher · View at Google Scholar · View at Scopus
  38. R. C. Block, E. R. Dorsey, C. A. Beck, J. T. Brenna, and I. Shoulson, “Altered cholesterol and fatty acid metabolism in Huntington disease,” Journal of Clinical Lipidology, vol. 4, no. 1, pp. 17–23, 2010. View at Publisher · View at Google Scholar · View at Scopus
  39. M. Doria, L. Maugest, T. Moreau, G. Lizard, and A. Vejux, “Contribution of cholesterol and oxysterols to the pathophysiology of Parkinson’s disease,” Free Radical Biology & Medicine, vol. 101, pp. 393–400, 2016. View at Publisher · View at Google Scholar · View at Scopus
  40. N. D. Perera and B. J. Turner, “AMPK signalling and defective energy metabolism in amyotrophic lateral sclerosis,” Neurochemical Research, vol. 41, no. 3, pp. 544–553, 2016. View at Publisher · View at Google Scholar · View at Scopus
  41. P. Prasanna, A. Thibault, L. Liu, and D. Samid, “Lipid metabolism as a target for brain cancer therapy: synergistic activity of lovastatin and sodium phenylacetate against human glioma cells,” The Journal of Neurochemistry, vol. 66, no. 2, pp. 710–716, 1996. View at Publisher · View at Google Scholar
  42. M. Tuohetahuntila, M. R. Molenaar, B. Spee et al., “ATGL and DGAT1 are involved in the turnover of newly synthesized triacylglycerols in hepatic stellate cells,” Journal of Lipid Research, vol. 57, no. 7, pp. 1162–1174, 2016. View at Publisher · View at Google Scholar · View at Scopus
  43. J. E. C. Sykes and M. Lopes-Cardozo, “Effect of exogenous fatty acids on lipid synthesis, marker-enzymes, and development of glial cells maintained in serum-free culture,” Glia, vol. 3, no. 6, pp. 495–501, 1990. View at Publisher · View at Google Scholar · View at Scopus
  44. F. Leonardi, L. Attorri, R. D. Benedetto et al., “Effect of arachidonic, eicosapentaenoic and docosahexaenoic acids on the oxidative status of C6 glioma cells,” Free Radical Research, vol. 39, no. 8, pp. 865–874, 2005. View at Publisher · View at Google Scholar · View at Scopus
  45. C. F. Semenkovich, “Regulation of fatty acid synthase (FAS),” Progress in Lipid Research, vol. 36, no. 1, pp. 43–53, 1997. View at Publisher · View at Google Scholar · View at Scopus
  46. F. Echeverría, M. Ortiz, R. Valenzuela, and L. A. Videla, “Hydroxytyrosol and cytoprotection: a projection for clinical interventions,” The International Journal of Biochemistry & Cell Biology, vol. 18, no. 5, article e930, 2017. View at Publisher · View at Google Scholar
  47. A. M. Giudetti, F. Damiano, G. V. Gnoni, and L. Siculella, “Low level of hydrogen peroxide induces lipid synthesis in BRL-3A cells through a CAP-independent SREBP-1a activation,” The International Journal of Biochemistry & Cell Biology, vol. 45, no. 7, pp. 1419–1426, 2013. View at Publisher · View at Google Scholar · View at Scopus
  48. L. Liu, K. Zhang, H. Sandoval et al., “Glial lipid droplets and ROS induced by mitochondrial defects promote neurodegeneration,” Cell, vol. 160, no. 1-2, pp. 177–190, 2015. View at Publisher · View at Google Scholar · View at Scopus
  49. W. Shao and P. J. Espenshade, “Expanding roles for SREBP in metabolism,” Cell Metabolism, vol. 16, no. 4, pp. 414–419, 2012. View at Publisher · View at Google Scholar · View at Scopus
  50. F. Damiano, A. Rochira, R. Tocci, S. Alemanno, A. Gnoni, and L. Siculella, “HnRNP A1 mediates the activation of the IRES-dependent SREBP-1a mRNA translation in response to endoplasmic reticulum stress,” Biochemical Journal, vol. 449, no. 2, pp. 543–553, 2013. View at Publisher · View at Google Scholar · View at Scopus
  51. F. Visioli, C. Galli, F. Bornet et al., “Olive oil phenolics are dose-dependently absorbed in humans,” FEBS Letters, vol. 46, no. 2-3, pp. 159-160, 2000. View at Publisher · View at Google Scholar · View at Scopus
  52. R. de la Torre, “Bioavailability of olive oil phenolic compounds in humans,” Inflammopharmacology, vol. 16, no. 5, pp. 245–247, 2008. View at Publisher · View at Google Scholar · View at Scopus
  53. F. Visioli and C. Galli, “Olive oil: more than just oleic acid,” The American Journal of Clinical Nutrition, vol. 72, no. 3, p. 853, 2000. View at Google Scholar
  54. E. Miro-Casas, M. I. Covas, M. Farre et al., “Hydroxytyrosol disposition in humans,” Clinical Chemistry, vol. 49, no. 6, pp. 945–952, 2003. View at Publisher · View at Google Scholar · View at Scopus
  55. M. I. Covas, K. de la Torre, M. Farré-Albaladejo et al., “Postprandial LDL phenolic content and LDL oxidation are modulated by olive oil phenolic compounds in humans,” Free Radical Biology & Medicine, vol. 40, no. 4, pp. 608–616, 2006. View at Publisher · View at Google Scholar · View at Scopus