4-Hydroxynonenal Contributes to Angiogenesis through a Redox-Dependent Sphingolipid Pathway: Prevention by Hydralazine Derivatives

Figure 1

4-HNE is colocalized with CD31 in human atherosclerotic lesions. Paraffin sections of human carotid plaques from endarterectomy were analyzed. In (a), hematoxylin/eosin (H/E) staining and immunostaining for 4-HNE-adduct (HNE) and CD68 expression. In (b), immunofluorescence analysis of 4-HNE-adduct expression (green) and CD31 (red), with nuclei counterstaining by DAPI. Int: intima. These pictures are representative of analysis for 3 separate advanced carotid plaques.

(a)

(b)