Arsenite Effects on Mitochondrial Bioenergetics in Human and Mouse Primary Hepatocytes Follow a Nonlinear Dose Response

Figure 1

Nonlinear dose response of arsenite in primary hepatocytes. Growth and survival response of (a) mouse primary hepatocytes, (b) human primary hepatocytes, and (c) human hepatoma cell line HepG2 exposed to increasing concentration of sodium arsenite for 16 h. (d) Arsenite IC₅₀ values for HepG2, mouse primary hepatocytes, and human primary hepatocytes. Growth and survival were measured using the MTT assay as described in methods. Results are representative of three independent experiments. The insets in (a), (b), and (c) show the toxicity profile at arsenite concentrations from 0 to 10 μM. Values represent mean ± SD. Survival values were significantly different from vehicle treated control cells; .

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