Oxidative Medicine and Cellular Longevity / 2017 / Article / Fig 6

Research Article

Apocynin Prevents Abnormal Megakaryopoiesis and Platelet Activation Induced by Chronic Stress

Figure 6

Apocynin partially reduces megakaryocyte alteration induced by chronic stress. (a) Hematoxylin and Eosine (H&E) staining of bone marrow from control mice (treated with vehicle) and from mice exposed to forced swimming stress with vehicle (FSS) or with Apocynin (FSS + Apocynin). Circle indicates MKs. (b) Quantification of panel (a) expressed as megakaryocytes per filed (40x magnification). Flow cytometric analyses: (c) percentage of CD41+/CD61+ cells and (f) percentage of CD41+/CD42d+ cells in bone marrow of mice. Analysis of (d) area and of (e) nuclear complexity in megakaryocytes from control, stress, and stress + Apocynin mice. mice/group. , , and ns = nonsignificant. For each box-plot, the center line illustrates the median and box limits indicate the 25th and 75th percentiles. Data were analyzed by the Kruskal-Willis test followed by a Dunn’s multiple comparison test.
(a)
(b)
(c)
(d)
(e)
(f)

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.