Apocynin Prevents Abnormal Megakaryopoiesis and Platelet Activation Induced by Chronic Stress

Figure 6

Apocynin partially reduces megakaryocyte alteration induced by chronic stress. (a) Hematoxylin and Eosine (H&E) staining of bone marrow from control mice (treated with vehicle) and from mice exposed to forced swimming stress with vehicle (FSS) or with Apocynin (FSS + Apocynin). Circle indicates MKs. (b) Quantification of panel (a) expressed as megakaryocytes per filed (40x magnification). Flow cytometric analyses: (c) percentage of CD41⁺/CD61⁺ cells and (f) percentage of CD41⁺/CD42d⁺ cells in bone marrow of mice. Analysis of (d) area and of (e) nuclear complexity in megakaryocytes from control, stress, and stress + Apocynin mice. mice/group. , , and ns = nonsignificant. For each box-plot, the center line illustrates the median and box limits indicate the 25th and 75th percentiles. Data were analyzed by the Kruskal-Willis test followed by a Dunn’s multiple comparison test.

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