Apocynin Prevents Abnormal Megakaryopoiesis and Platelet Activation Induced by Chronic Stress

Figure 7

Apocynin prevents oxidative stress in megakaryocytes of stressed mice. Control mice and mice exposed to forced swimming stress (FSS) were treated with vehicle or with Apocynin (FSS + Apocynin), and then (a) NADPH/NADP⁺ ratio was measured in bone marrow. (b) Immunoperoxidase staining of NOX1 was performed in bone marrow. (c) Reactive oxygen species (ROS) levels were detected by H2DCF-DA in MKs, as described in Methods. mice/group. and . For each box-plot, the center line illustrates the median and box limits indicate the 25th and 75th percentiles. Data were analyzed by the Kruskal-Willis test followed by a Dunn’s multiple comparison test.

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