Figure 5: Analysis of mitochondria network. (a) Representative images showing mitochondrial morphological skeleton obtained with MiNa toolset [31] of healthy and SAD fibroblasts untreated (control), after 6 h of CCCP treatment followed by 1 h without it (reversion) or after 7 h with 20 μM CCCP (total CCCP). (b)–(e) Quantification of number of individuals (b), networks (c), branches per network (d), or length of the branches (e) of different healthy/AD sex- and age-matched couple samples: AG11362/AG05809, AG07310/AG06869, and AG11362/AG05809. ; ns: not significant.