Ginkgolide B Suppresses TLR4-Mediated Inflammatory Response by Inhibiting the Phosphorylation of JAK2/STAT3 and p38 MAPK in High Glucose-Treated HUVECs

Figure 1

Ginkgolide B inhibits TLR4 expression in high glucose- and LPS-treated HUVECs. The cells were treated with various concentrations of ginkgolide B (GB) for 1 h, and glucose (30 mM) or LPS (1 μg/ml) was then added for 8 h. (a) Ginkgolide B suppressed TLR4 RNA expression that was induced by high glucose. (b) Ginkgolide B decreased TLR4 protein expression that was induced by high glucose. (c) Ginkgolide B decreased MyD88 expression that was induced by high glucose. (d) TLR4 expression was detected by immunofluorescence in high glucose-treated HUVECs. (e) Ginkgolide B suppressed TLR4 expression that was induced by LPS. The data were obtained from four independent experiments. The column chart represents the density analysis from four independent experiments. ^#, significant difference between nonhigh glucose-treated cells and high glucose cells (a–c) or between non-LPS-treated cells and LPS-treated cells (e); , significant difference between non-ginkgolide B-treated cells and ginkgolide B-treated cells.

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