Antihypertensive Effects of Roselle-Olive Combination in L-NAME-Induced Hypertensive Rats

Table 5

Real-time PCR quantitation of eNOS mRNA expression in the kidney and heart of the different experimental groups.

Groups	Fold change (RQ) in the kidney	Fold change (RQ) in the heart
Group 1	1^ǂ	1^ǂ
Group 2	0.14 ± 0.09	0.16 ± 0.08
Group 3	3.05 ± 1.12^ǂ	6.67 ± 3.33^ǂ
Group 4	3.97 ± 1.80^ǂ	12.9 ± 4.60^ǂ
Group 5	0.73 ± 0.11^ǂ	10.11 ± 3.15^ǂ
Group 6	0.16 ± 0.06	3.43 ± 2.83^ǂ

Group 1: normal control; group 2: hypertensive control (L-NAME 50 mg/kg); group 3: L-NAME + lisinopril (10 mg/kg); group 4: L-NAME + Roselle-Olive (500 mg/kg); group 5: L-NAME + Roselle-Olive (250 mg/kg); group 6: L-NAME + Roselle-Olive (125 mg/kg). Values represent fold increases in mRNA level over the control group. GAPDH was used as an invariant internal control for calculating mRNA-fold changes. Values are expressed as mean ± SE. , statistically significant from the normal control group at the corresponding time. , statistically significant from the hypertensive control group at the corresponding time.