Cytoprotective Effects of Cell-Permeable Bifunctional Antioxidant Enzyme, GST-TAT-SOD, against Cisplatin-Induced Cell Damage

Figure 3

DCFH-DA detection of total ROS in L-02 cells treated with cisplatin. Cells were plated in triplicate, pretreated with or without proteins, and treated with 0.7 μg/mL cisplatin for 24 h. After incubation, cells were washed with PBS and incubated with 10 μM DCFH-DA for 0.5 h at 37°C. Fluorescence was corrected for background signal and normalized for protein content and expressed as fluorescence/mg of protein. The bars indicate the means ± SD (). The CDDP group was compared with the control group (). The pretreated group was compared with the CDDP group ().