Spontaneous Production of Glutathione-Conjugated Forms of 1,2-Dichloropropane: Comparative Study on Metabolic Activation Processes of Dihaloalkanes Associated with Occupational Cholangiocarcinoma

Figure 2

Effect of GSTT1 on the reactivity of halogenated hydrocarbon with GSH. (a) Data-processing scheme for differential analysis of four cases: in vitro reaction of 1,2-DCP or DCM with GSH in the presence or absence of GSTT1. The seven component products are summarized in Table 1. (b) Principal component analysis (PCA) based on differentially detected peaks across incubation component conditions. Green, 1,2-DCP without GSTT1; red, 1,2-DCP with GSTT1; purple, DCM without GSTT1; and yellow, DCM with GSTT1. This PCA of acquired data, such as peak information including integrated intensity, was performed by using the SIEVE 2.1 program. Upper panel, the first second-principal components; lower panel, the third two-principal components of all extracted data are plotted. in each group.

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