Oxidative Medicine and Cellular Longevity / 2017 / Article / Fig 4

Research Article

Salidroside Inhibits HMGB1 Acetylation and Release through Upregulation of SirT1 during Inflammation

Figure 4

Salidroside inhibits the translocation of HMGB1 from the nucleus to the cytoplasm induced by LPS in RAW264.7 cells. Cells were pretreated with salidroside for 2 h and then treated with LPS for 12 h. (a) Nuclear and cytoplasmic proteins were extracted, respectively, and Western blotting was used to determine the amounts of nuclear and cytoplasmic HMGB1. (b) After treatment, the cells in laser confocal dishes were fixed with 4% paraformaldehyde for 30 min, blocked with 3% BSA for 1 h, incubated with an anti-HMGB1 antibody overnight, and then incubated with an Alexa Fluor 555 goat anti-rabbit IgG (red) antibody for 1 h in the dark. Cell nuclei were stained with DAPI (blue) for 3 min, and the localization of HMGB1 in cells was visualized by confocal microscopy.

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