Thioredoxin-1 Protects Bone Marrow-Derived Mesenchymal Stromal Cells from Hyperoxia-Induced Injury In Vitro

Figure 1

Characterization of rat bone marrow-derived mesenchymal stromal cells (BMSCs). (a) The plastic-adherent cells demonstrated a homogeneous fibroblast-like and spindle-shaped morphology. Original magnification, ×100. (b) Adipogenic differentiation of BMSCs stained with oil red O. Original magnification, ×200. (c) Osteogenic differentiation of BM-MSCs stained with alizarin red. Original magnification, ×400. (d) FACS analysis demonstrated expression of markers attributed to BMSCs. The cells were devoid of hematopoietic cells as indicated by the lack of CD45 and CD34. The MSC-specific markers, CD29, CD44, CD73, CD105, and CD90 were strongly expressed on the cells.

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