Vialinin A, an Edible Mushroom-Derived p-Terphenyl Antioxidant, Prevents VEGF-Induced Neovascularization In Vitro and In Vivo

Figure 4

Effect of vialinin A on VEGF-induced oxidative stress in HUVEC. Growth-arrested HUVECs were pretreated with vialinin A followed by treatment without/with VEGF (10 ng/mL) overnight. The cells were stained with CM-H2DCFDA for 20 min and analyzed with a flow cytometer (BD LSRII Fortessa). (a) Histograms showing the effect of vialinin A on VEGF-induced ROS production in HUVECs (red: VEGF, blue: VEGF + vialinin A, light blue: untreated control, pink: vialinin A only, and grey solid line: unstained control). (b) Data were presented as fold change of mean fluorescence intensity (MFI) compared to unstained control analyzed using Flow Jo software. (c) MDA levels were determined with a MDA assay kit from Oxis Research. Values are the mean ± SD (). and when compared to untreated control; ^## when compared to VEGF treated.

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