Changes of KEAP1/NRF2 and IKB/NF-κB Expression Levels Induced by Cell-Free DNA in Different Cell Types
Expression level of BCL2 gene (a) and BCL2, BCL2A1, BCL2L1, BIRC2 (c-IAP1), and BIRC3 (c-IAP2) genes (b) after an exposure of MSC to oxidized and nonoxidized GC-rich cfDNA fragments (50 ng/ml, see the exposure duration in the figure). Averaged values of three independent tests on MSC cultures derived from three different donors are shown. As an internal standard, ТВР gene was used. (c) Expression of BCL2, BCL2A1, and BCL2L1 genes in MSC cultures with heterozygous mutations in BRCA1 (5382insC) and TP53 (L145p) genes. Values are significantly different from the control (, Mann–Whitney U test). gDNA: genomic DNA; p(rDNA): ribosomal repeat.