Effects of Post Ischemia-Reperfusion Treatment with Trimetazidine on Renal Injury in Rats: Insights on Delayed Renal Fibrosis Progression
Effects of postischemia-reperfusion (I/R) treatment with TMZ on the expressions of HIF-1α and VEGF. Western blot analysis (a) and densitometric analysis (b, c) of the Western blot for HIF-1α and VEGF at 24 h, 5 d, and 8 w after I/R injury, respectively. Renal I/R injury induced increases in HIF-1α levels and decreases in VEGF levels in the IRC groups compared to that in the sham groups at 24 h and 5 d after I/R injury. Post I/R treatment with TMZ significantly increased both levels of HIF-1α and VEGF compared to the IRC groups at 24 h and 5 d after I/R injury. HIF-1α could not be detected in any groups at 8 w after I/R injury. At 8 w after I/R injury, VEGF levels were all significantly higher in the IRC and TMZ-treated groups compared to that in the sham groups without any intergroup differences between the IRC and the TMZ-treated groups. Sham = rats not underwent I/R; IRC = rats underwent ischemia (45 min)-reperfusion (24 h, 5 d, and 8 w); TMZS = rats treated with TMZ (3 mg/kg) upon reperfusion; TMZD = rats treated with TMZ (3 mg/kg) once daily for 5 d or 8 w starting upon reperfusion. versus sham, versus IRC.