Research Article
The Critical Role of IL-10 in the Antineuroinflammatory and Antioxidative Effects of Rheum tanguticum on Activated Microglia
Figure 5
The inhibitory effects of Rt on the CGA-induced expression of proinflammatory and oxidative mediators in microglia. (a–c) The effect of Rt (10 μg/ml) on the mRNA expression of TNF-α (a), IL-1β (b), and iNOS (c) in the MG6 cells after stimulation with CGA. Each column and bar represents the mean ± SEM ( each). The asterisks indicate a statistically significant difference from the indicated value (, ). (d) The secretion of IL-1β in the MG6 cells 96 h after stimulation with CGA with or without pretreatment with Rt. The results represent the mean ± SEM of three independent experiments. The asterisks indicate a statistically significant difference from the indicated value (). (e) The examination of NO metabolites in the culture medium of MG6 cells 72 h after stimulation with CGA with or without pretreatment with Rt by NO2−/NO3− assay. The results represent the mean ± SEM of three independent experiments. The asterisks indicate a statistically significant difference from the indicated value. (). (f) The effect of Rt (10 μg/ml) on the mRNA expression of IL-10 in the MG6 cells after stimulation with CGA. The results represent the mean ± SEM of four independent experiments. The asterisks indicate a statistically significant difference from the indicated value ().
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