Research Article

The Critical Role of IL-10 in the Antineuroinflammatory and Antioxidative Effects of Rheum tanguticum on Activated Microglia

Figure 6

The capable regulation of IL-10 by Rt in MG6 cells and primary cultured microglia. (a) The mRNA changes of IL-10 at different time points after treatment with Rt (10 μg/ml). The results represent the mean ± SEM of three independent experiments. The asterisks indicate a statistically significant difference from the value at the start of experiments (0 h) (, ). (b) The mRNA changes of IL-10 24 h after treatment with different doses of Rt. The results represent the mean ± SEM of three independent experiments. The asterisks indicate a statistically significant difference from the value in untreated cells (). (c) The secretion of IL-10 in the cultured medium of MG6 cells at different time points after treatment with Rt (10 μg/ml). The results represent the mean ± SEM of three independent experiments. The asterisks indicate a statistically significant difference from the value at the start of experiments (0 h) (). (d) The secretion of IL-10 in the cultured medium of MG6 cells 24 h after treatment with different doses of Rt. The results represent the mean ± SEM of three independent experiments. The asterisks indicate a statistically significant difference from the value in untreated cells (). (e) The secretion of IL-10 in the cultured medium of primary cultured microglia 24 h after treatment with Rt (10 μg/ml). The results represent the mean ± SEM of three independent experiments. The asterisks indicate a statistically significant difference from the value in untreated cells ().
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