The Critical Role of IL-10 in the Antineuroinflammatory and Antioxidative Effects of Rheum tanguticum on Activated Microglia

Figure 8

Effects of Rt on the NF-κB activation pathways in CGA-stimulated microglia. (a) Immunofluorescence CLMS images indicating the nuclear translocation of p65 (green) in MG6 cells with Hoechst-stained nuclei (blue) at 24 h after stimulation with CGA (10 nM) in the presence or absence of Rt (10 μg/ml) and IL-10NAb. Scale bar, 15 μm. The typical cells were analyzed by line plot profile to show the cytosol and nuclear location of p65. (b) The protein expression of phospho-IκBα at different time points after stimulation with CGA. (c) The protein expression of phospho-IκBα in MG6 cells 30 min after CGA stimulation in the presence or absence of Rt (10 μg/ml) and IL-10NAb and the quantitative analyses of the immunoblotting for phospho-IκBα. The results represent the mean ± SEM of four independent experiments. The asterisks indicate a statistically significant difference from the indicated value (, ).

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