Intermittent Hypoxia Increases the Severity of Bleomycin-Induced Lung Injury in Mice

Figure 3

Intermittent hypoxia increases lung inflammation and pulmonary edema in bleomycin-treated mice. (a) Total cell count was determined in BAL from mice at day 4 in the 4 experimental groups. Changes in BAL cell population (Macro: macrophages; PMN: polymorphonuclear cells; Ly: lymphocytes) were determined in the 4 experimental groups. Data represent means ± SE, mice per group. (b) Myeloperoxidase (MPO) concentration was assessed by ELISA in lung homogenate of mice at day 4 and day 8 in the 4 experimental groups. Data represent means ± SE, mice per group. (c) Lung histology of mice at day 8. Lung sections were prepared at day 8 in the 4 experimental groups and stained with haematoxylin and eosin to assess lung morphology. (d) Lung wet/dry weight ratios for all experimental groups at day 8. Data represent means ± SE, mice per group. versus Saline-IA; versus Saline-IA; versus Saline-IA; versus BLM-IA; versus BLM-IA; versus BLM-IA.

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