Research Article

Losartan Ameliorates Calcium Oxalate-Induced Elevation of Stone-Related Proteins in Renal Tubular Cells by Inhibiting NADPH Oxidase and Oxidative Stress

Figure 6

Losartan downregulated NF-κB pathway activity and stone-related protein expression by attenuation of renal tubular cell oxidative stress. (a) NRK-52E cells were induced by COM (1 mM) for 6 h with or without losartan preincubation, and then intracellular ROS was detected by flow cytometry. The expression of NADPH oxidase subunits (Nox2 and Nox4) (b), NF-κB subunits (p50 and p65) (c), and stone-related proteins (OPN, CD44, and MCP-1) (d) was decreased in the COM + Ang II + losartan group compared with the COM + Ang II group in NRK-52E cells. (e) Losartan administration ameliorated the CaOx depositions (black arrow) compared with the rat kidneys of the hyperoxaluria group (Von Kossa staining, magnification: ×400). The data are expressed as mean ± SEM. compared with the Ang II group and compared with the COM + Ang II group. compared with the Ang II group and the COM + Ang II group.
(a)
(b)
(c)
(d)
(e)