Intracellular and Intercellular Signalling Mechanisms following DNA Damage Are Modulated By PINK1
PINK1 loss of function leads to the induction of an adaptive response by BLM in MEFs. (a) Design of adaptive response experiments. The cells were exposed to low-dose genotoxic treatment (4 μg/mL BLM) and incubated for 24 hours with fresh medium. The cells were treated again with a higher dose of BLM (40 μg/mL). The genotoxicity was analyzed 24 h later for all the treatments 4 μg/mL, 40 μg/mL, and 4 + 40 μg/mL. A reduction in genotoxicity at the combined treatment of 4 + 40 μg/mL compared to the 40 μg/mL treatment signifies an adaptive response. (b, c) WT MEFs do not respond adaptively while the PINK1-KO MEFs show an adaptive response following these treatments. Each data point represents the mean ± SEM of at least three independent experiments. Statistical analysis is performed with one-way ANOVA with multiple comparisons, and the significance level is indicated in the figure. , and .
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