Intracellular and Intercellular Signalling Mechanisms following DNA Damage Are Modulated By PINK1

Figure 5

PINK1 loss of function leads to the induction of an adaptive response by BLM in MEFs. (a) Design of adaptive response experiments. The cells were exposed to low-dose genotoxic treatment (4 μg/mL BLM) and incubated for 24 hours with fresh medium. The cells were treated again with a higher dose of BLM (40 μg/mL). The genotoxicity was analyzed 24 h later for all the treatments 4 μg/mL, 40 μg/mL, and 4 + 40 μg/mL. A reduction in genotoxicity at the combined treatment of 4 + 40 μg/mL compared to the 40 μg/mL treatment signifies an adaptive response. (b, c) WT MEFs do not respond adaptively while the PINK1-KO MEFs show an adaptive response following these treatments. Each data point represents the mean ± SEM of at least three independent experiments. Statistical analysis is performed with one-way ANOVA with multiple comparisons, and the significance level is indicated in the figure. , and .

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