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Oxidative Medicine and Cellular Longevity
Volume 2018, Article ID 1538632, 10 pages
https://doi.org/10.1155/2018/1538632
Research Article

Antiaging of Cucurbitane Glycosides from Fruits of Momordica charantia L.

College of Pharmaceutical Sciences, Zhejiang University, Yu Hang Tang Road 866, Hangzhou 310058, China

Correspondence should be addressed to Lan Xiang; nc.ude.ujz@gnaixl and Jianhua Qi; nc.ude.ujz@auhnaijiq

Received 10 September 2017; Revised 21 December 2017; Accepted 11 January 2018; Published 25 March 2018

Academic Editor: Carolina G. Llorente

Copyright © 2018 Xueli Cao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Methanol extracts of Momordica charantia L. fruits are extensively studied for their antiaging activities. A new cucurbitane-type triterpenoid (1) and nine other known compounds (2–10) were isolated, and their structures were determined according to their spectroscopic characteristics and chemical derivatization. Biological evaluation was performed on a K6001 yeast bioassay system. The results indicated that all the compounds extended the replicative lifespan of K6001 yeast significantly. Compound 9 was used to investigate the mechanism involved in the increasing of the lifespan. The results indicated that this compound significantly increases the survival rate of yeast under oxidative stress and decreases ROS level. Further study on gene expression analysis showed that compound 9 could reduce the levels of UTH1 and SKN7 and increase SOD1 and SOD2 gene expression. In addition, it could not extend the lifespan of the yeast mutants of Uth1, Skn7, Sod1, and Sod2. These results demonstrate that compound 9 exerts antiaging effects via antioxidative stress and regulation of UTH1, SKN7, SOD1, and SOD2 yeast gene expression.