Nonylphenol and Octylphenol Differently Affect Cell Redox Balance by Modulating the Nitric Oxide Signaling

Figure 2

(a) eNOS and iNOS protein expression and (b) evaluation of eNOS uncoupling, in response to OP and NP. HepG2 were incubated 24 h with OP and NP, 10 and 20 μM, respectively, and assayed by Western blot. (a) eNOS and iNOS protein detection was carried out using isoform-specific anti-eNOS and anti-iNOS antibodies. Values are reported as fold increase versus the protein expressed by control cells (CTR) (data ± SEM, ). values were considered statistically significant by ANOVA. (b) Phosphorylation at Ser1177 and Thr495 of eNOS, determined using anti p-Ser1177 and anti p-Thr495 eNOS-specific antibodies. Values are reported as the ratio of phospho eNOS (Ser 1177 or Thr 495) over total eNOS (data ± SEM, ). values were considered statistically significant by ANOVA. Inset: typical Western blot pattern as detected using antibodies against iNOS, eNOS, p-S1177 eNOS, and p-T495 eNOS, and following 24 h cell incubation with OP and NP; α-tubulin as reference (details in Methods).

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