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Figure 8: Role of VDR in the inhibitory effects of vitamin D treatment on Pin1 protein expression and activity in high glucose-cultured HUVECs. Total proteins were extracted after VDR-specific siRNA transfection for 72 h for immunoblotting analysis. Pin1 protein expression levels of HUVECs were expressed as the ratio of Pin1 over β-actin. Pin1 activity of HUVEC lysate was measured by using a commercially available kit. HG: high glucose (33 mM); results are presented as mean ± SEM (); versus control; versus HG (33 mM); versus HG (33 mM) + VitD (10−6 M) + scrambled siRNA.