Downregulated Expression of Solute Carrier Family 26 Member 6 in NRK-52E Cells Attenuates Oxalate-Induced Intracellular Oxidative Stress

Figure 6

Less Slc26a6 expression in kidney protects rats from urolithiasis. After renal capsular transfection for 2 weeks, rats were treated with 1% EG and 0.5% NH₄Cl. (a, b) IHC assay indicates that lentivirus-siRNA reduced the Slc26a6 expression in the kidney but not in the duodenum (). Magnification, ×200, . (c) DHE staining was used to detect SOD generation, and the siRNA group showed higher decreases in SOD production than other groups did. Magnification, ×200, . (d) Representative micrographs showing TUNEL staining images of different groups (). Magnification, ×200, . (e) von Kossa staining to detect crystal formation and the siRNA group formed least crystals in the kidney. Arrows indicate crystals (). Magnification, ×200, .

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