Propofol Protects Hippocampal Neurons from Hypoxia-Reoxygenation Injury by Decreasing Calcineurin-Induced Calcium Overload and Activating YAP Signaling

Figure 1

Propofol attenuated hypoxia-reoxygenation, suppressed viability, and induced apoptosis of HT-22 cells. (a, b, and c) HT-22 cells were pretreated without or with different concentrations of propofol for 2 hr prior to stimulation with hypoxia-reoxygenation (H/R). Cell viability was analyzed by MTT (a). Cell apoptosis was tested by TUNEL assay (b) and flow cytometry cell apoptosis assay (c, 15 μmol/l propofol). (d) Expression levels of Bax, caspase-3 (cleaved), and caspase-9 in HT-22 cells with different treatments were analyzed by Western blots. (^aDifferences were significant when compared with “control,” . ^bDifferences were significant when compared with “H/R,” . ^cDifferences were significant when compared with “H/R + 5 μmol/l,” . ^dDifferences were significant when compared with “H/R + 10 μmol/l,” .)

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