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Oxidative Medicine and Cellular Longevity
Volume 2018, Article ID 1780956, 10 pages
https://doi.org/10.1155/2018/1780956
Research Article

Anti-Inflammatory, Immunomodulatory, and Antioxidant Activities of Allicin, Norfloxacin, or Their Combination against Pasteurella multocida Infection in Male New Zealand Rabbits

1Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511 Sharkia, Egypt
2Department of Internal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511 Sharkia, Egypt
3Biochemistry Department, Faculty of Science-Al Faisaliah, King Abdulaziz University, Jeddah, Saudi Arabia
4Bourgogne Franche-Comté University, Chrono-Environnement Laboratory, UMR CNRS 6249, 25030 Besançon Cedex, France
5Department of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt

Correspondence should be addressed to Mohamed M. Abdel-Daim; ge.ude.zeus.tev@m.miadledba

Received 15 February 2018; Accepted 8 April 2018; Published 27 June 2018

Academic Editor: Mansur A. Sandhu

Copyright © 2018 Rasha T. M. Alam et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study investigated the efficacy of allicin as an antibacterial, anti-inflammatory, antioxidant, and immunostimulant agent in reducing the severity of Pasteurella multocida (P. multocida) type B infection in rabbits. Fifty New Zealand rabbits, 5 weeks old, were divided equally into five groups. Except for group 1, all groups were intranasally infected with P. multocida type B (2 × 105 colony forming units/ml/rabbit). Then, group 3 rabbits were orally treated with allicin (50 mg/kg BW) for 5 days, group 4 rabbits received a single oral dose of norfloxacin 30% (100 mg/kg BW), while group 5 rabbits were treated with a combination of norfloxacin and allicin. Hematological, serum biochemical, inflammatory cytokine, immunological, and histopathological analyses were performed. Results revealed that rabbits, infected with P. multocida type B, exhibited macrocytic hypochromic anemia and leukocytosis with a significant elevation in the phagocytic percentage and index. Moreover, significant reductions in serum total protein, albumin, globulin, and immunoglobulin (IgG and IgM) levels were observed in infected rabbits. Infected rabbits showed significant increases in serum inflammatory cytokine (TNF-α and IL-6), alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, and serum bilirubin (total, direct, and indirect) levels. Further, P. multocida infection induced oxidative stress as demonstrated by the significant reduction in serum levels of reduced glutathione and superoxide dismutase enzyme and marked elevation in serum malondialdehyde. Treatment with allicin, norfloxacin, or their combination significantly ameliorated the alterations in all studied parameters. In conclusion, allicin could ameliorate the inflammation and oxidative stress, induced by P. multocida type B infection in rabbits.