Pulmonary Arterial Hypertension and Endothelial Dysfunction Is Linked to NADPH Oxidase-Derived Superoxide Formation in Venous Thrombosis and Pulmonary Embolism in Mice
Repeated thrombin injection led to recurrent PE and sustained PA pressure elevation and endothelial dysfunction. While after a single thrombin injection, symptoms were generally resolved and PA pressure normalized within minutes, the time to recovery lengthened with every injection. 24 h after (triple) pulmonary embolisms, PAT remained significantly shortened, indicating a persisting elevation of pulmonary pressure ((a) are presented as PAT (ms) and as calculated RVSP (mmHg), with a cutoff of 21 ms (PAT)/32 mmHg (RVSP) between normal and elevated PAP, with the grey area indicating the regions of elevated PAP ). 5 animals per group. Data are presented as mean and SEM. 1-way ANOVA and Bonferroni’s multiple comparison test. (b) Isometric tension studies revealed a significant endothelial dysfunction as compared to untreated mice in PAs explanted from embolised animals. APE: acute PE; PAT: pulmonary arterial acceleration time. 5 animals per group. Data are presented as mean and SEM. 2-way ANOVA and Dunn’s multiple comparison test.
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.