Oxidative Medicine and Cellular Longevity / 2018 / Article / Fig 3

Research Article

Cell Size Influences the Reproductive Potential and Total Lifespan of the Saccharomyces cerevisiae Yeast as Revealed by the Analysis of Polyploid Strains

Figure 3

Mean cell size in a population of yeast cells differing in ploidy. (a) Shape and size diversity of yeast cells differing in ploidy. (b) Volume of yeast cells differing in ploidy. Cell volume was estimated by analysis of microscopic images. Data are presented as the mean values for at least 100 cells from each yeast strain. The bars indicate SD from all cells tested in two independent experiments. The stars indicate values that are significantly different from values obtained for the haploid strain (1n) within the same genetic background using one-way ANOVA and Dunnett’s post hoc test for SP4 and BY474X strains or t-test for BMA64 strain; . (c) Cell size of the yeast strains in the SP4, BY474X, and BMA64 backgrounds as measured by forward scatter (FSC histogram reflects the cells size in the population). The cells were analyzed via FACS as described in the Materials and Methods. Histograms were obtained for 10,000 cells per strain.

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