Isobavachalcone Induces ROS-Mediated Apoptosis via Targeting Thioredoxin Reductase 1 in Human Prostate Cancer PC-3 Cells

Figure 6

IBC-induced ER stress was mediated by ROS generation. PC-3 cells were treated with 45 μM IBC with or without 5 mM NAC or 1 mM BSO for 24 h, and then ER stress marker expression levels were measured by Western blot. (a) Protein levels of GRP78, ATF4, Chop, and p-eIF2α were examined by Western blot. (b) Quantitative analysis of GRP78, ATF4, Chop, and p-eIF2α protein levels. All data are presented as mean ± S.D. and compared to the control group.

(a)

(b)